Advanced Concepts in Protein Science

Advanced Concepts in Protein Science

Central dogma of biochemistry and the importance of proteins

• molecular cloning and protein engineering

• protein expression and purification 

Protein structure and stability

• from amino acids to higher levels of organization 

• protein denaturation 

• energetics of protein structure formation 

• double mutant cycles 

Practical methods 1

• optical spectroscopy 

• kinetics of protein-ligand interactions 

Journal club

• DNA replication

• the ribosome and protein biosynthesis 

Protein folding and energy landscapes

• protein folding kinetics 

• microscopic and macroscopic constants 

• folding pathways 

• molecular chaperones 

Journal club

• molecular chaperones

  • GroEL/ES

  • Hsp90

• posttranslational modification – Ubiquitinylation and SUMOylation 

• protein degradation – the proteasome 

Membrane proteins

• structure and function of biological membranes

• working with membrane proteins 

• membrane mimetic systems 

G-protein coupled receptors

• architecture 

• conformational changes during signal transduction 

Ion channels

• taxonomy and evolution  

• basics of ion selectivity

• the Hodgkin-Huxley model 

• ion channel gating

Journal club

• protein structure prediction

Practical methods 2

• single channel electrophysiology

• cryogenic electron microscopy (cryo-EM)

Journal club

• voltage sensing domains

• TRPV1 – the capsaicin receptor

Ion channel physiology

• regulation by cellular components

• channelopathies

Journal club

• olfactory receptors

• proteins as tools - targeted drug delivery 

Copyright © 2023 Philipp Schmidpeter